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sybr primerscript mirna rt‒pcr kit  (TaKaRa)


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    Structured Review

    TaKaRa sybr primerscript mirna rt‒pcr kit
    Sybr Primerscript Mirna Rt‒Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primerscript+rt+pcr+kit/pmc11373458-77-1-6
    Average 86 stars, based on 1 article reviews
    sybr primerscript mirna rt‒pcr kit - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The receptor kinase OsANX limits precocious flowering and inflorescence over-branching and maintains pollen tube integrity in rice.
    Article Snippet: CrRLK1L subfamily members are involved in diverse growthand development-related processes in Arabidopsis.. However, the functions of their counterparts in rice are unknown.. Here, OsANX expression was detected in developing inflorescences, mature pollen grains, and growing pollen tubes, and it was localized to the plasma membrane in pollen grains and tobacco epidermal cells.

    Article Title: HIF-1A regulates cognitive deficits of post-stroke depressive rats.
    Article Snippet: Post-stroke depression (PSD) is a serious neuropsychiatric complication post stroke and leads to cognitive deficits.. This study was conducted to explore the molecular mechanism of hypoxia-inducible factor-1α (HIF-1A) in cognitive dysfunction in rats with PSD.. The rat model of PSD was established by middle cerebral artery occlusion, followed by 3 weeks of treatment with chronic unpredictable mild stress.

    Article Title: SNHG15-Mediated Localization of Nucleolin at the Cell Protrusions Regulates CDH2 mRNA Expression and Cell Invasion
    Article Snippet: Briefly, total RNA was isolated using TRIzol Reagent (Takara, Shiga, Japan). .. A total of 1 μg RNA was reverse transcribed to cDNA using a PrimerScript RT-PCR kit (Takara). .. The qPCR was conducted using a SYBR Green reaction mix (Vazyme, Nanjing, China).

    Article Title: lncRNAs ITFG2-AS1 and MMP25-AS1 have significant high-expression in gastric cancer patients as the potential diagnostic biomarkers: integrated bioinformatics and experimental investigation
    Article Snippet: RNA extraction, cDNA synthesis, and qRT-PCR experiment Total RNA was extracted from cells using Trizol reagent (TaKaRa Biotechnology, Dalian, China) according to the manufacturer’s protocol. .. Total RNA was reverse transcribed with a PrimerScript RT-PCR kit (Takara Biotechnology, Dalian, China). .. Real time qPCR was conducted using a standard SYBR Green PCR kit (Roche, Upper Bavaria, Germany) protocol with a CFX real-time instrument (Bio-rad, Hercules, CA, USA).

    Article Title: Development of a fluorescence isothermal recombinase polymerase amplification assay for rapid detection of feline calicivirus.
    Article Snippet: Feline calicivirus (FCV) is responsible for a highly contagious disease in domestic cats.. FCV may cause multiple symptoms and even death to the infected cats.. A simple and cost-effective real-time RPA assay was developed for rapid detection of FCV in clinical samples.

    Expressing:

    Article Title: The receptor kinase OsANX limits precocious flowering and inflorescence over-branching and maintains pollen tube integrity in rice.
    Article Snippet: CrRLK1L subfamily members are involved in diverse growthand development-related processes in Arabidopsis.. However, the functions of their counterparts in rice are unknown.. Here, OsANX expression was detected in developing inflorescences, mature pollen grains, and growing pollen tubes, and it was localized to the plasma membrane in pollen grains and tobacco epidermal cells.

    Article Title: MicroRNA-27a inhibits porcine type I muscle fibre gene expression by directly targeting peroxisome proliferator-activated receptor-γ coactivator-1α.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 32102584; Natural Science Foundation of Guangdong Province, Grant/Award Number: 2023A1515011112 Abstract MicroRNAs are one of the key determinants of muscle fibre development and phenotype in mammals.. The preliminary experiment implied that microRNA‐27a (miR‐27a) might involve in regulation of muscle fibre type composition of pigs.. Thereby, the present study aimed to confirm the regulatory effect of miR‐27a on porcine type I muscle fibre‐encoding gene (myosin heavy chain gene 7, MYH7) expression and its related mechanism.

    Quantitative RT-PCR:

    Article Title: The receptor kinase OsANX limits precocious flowering and inflorescence over-branching and maintains pollen tube integrity in rice.
    Article Snippet: CrRLK1L subfamily members are involved in diverse growthand development-related processes in Arabidopsis.. However, the functions of their counterparts in rice are unknown.. Here, OsANX expression was detected in developing inflorescences, mature pollen grains, and growing pollen tubes, and it was localized to the plasma membrane in pollen grains and tobacco epidermal cells.

    Reverse Transcription:

    Article Title: HIF-1A regulates cognitive deficits of post-stroke depressive rats.
    Article Snippet: Post-stroke depression (PSD) is a serious neuropsychiatric complication post stroke and leads to cognitive deficits.. This study was conducted to explore the molecular mechanism of hypoxia-inducible factor-1α (HIF-1A) in cognitive dysfunction in rats with PSD.. The rat model of PSD was established by middle cerebral artery occlusion, followed by 3 weeks of treatment with chronic unpredictable mild stress.

    Article Title: SNHG15-Mediated Localization of Nucleolin at the Cell Protrusions Regulates CDH2 mRNA Expression and Cell Invasion
    Article Snippet: Briefly, total RNA was isolated using TRIzol Reagent (Takara, Shiga, Japan). .. A total of 1 μg RNA was reverse transcribed to cDNA using a PrimerScript RT-PCR kit (Takara). .. The qPCR was conducted using a SYBR Green reaction mix (Vazyme, Nanjing, China).

    Article Title: lncRNAs ITFG2-AS1 and MMP25-AS1 have significant high-expression in gastric cancer patients as the potential diagnostic biomarkers: integrated bioinformatics and experimental investigation
    Article Snippet: RNA extraction, cDNA synthesis, and qRT-PCR experiment Total RNA was extracted from cells using Trizol reagent (TaKaRa Biotechnology, Dalian, China) according to the manufacturer’s protocol. .. Total RNA was reverse transcribed with a PrimerScript RT-PCR kit (Takara Biotechnology, Dalian, China). .. Real time qPCR was conducted using a standard SYBR Green PCR kit (Roche, Upper Bavaria, Germany) protocol with a CFX real-time instrument (Bio-rad, Hercules, CA, USA).

    other:

    Article Title: Akirin2 enhances antibacterial ability via interacting with 14-3-3ζ in V. splendidus-challenged Apostichopus japonicus.
    Article Snippet: Akirin2 is pivotal for regulating host immunological responses in vertebrates, including antibacterial immunity and inflammation.. However, the functional significance of Akirin2 in invertebrates remains largely unexplored.. In this study, we cloned the complete cDNA sequence of Akirin2 from A. japonicus (AjAkirin2) and elucidated its immunological mechanism upon pathogen infection.

    Synthesized:

    Article Title: MicroRNA-27a inhibits porcine type I muscle fibre gene expression by directly targeting peroxisome proliferator-activated receptor-γ coactivator-1α.
    Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 32102584; Natural Science Foundation of Guangdong Province, Grant/Award Number: 2023A1515011112 Abstract MicroRNAs are one of the key determinants of muscle fibre development and phenotype in mammals.. The preliminary experiment implied that microRNA‐27a (miR‐27a) might involve in regulation of muscle fibre type composition of pigs.. Thereby, the present study aimed to confirm the regulatory effect of miR‐27a on porcine type I muscle fibre‐encoding gene (myosin heavy chain gene 7, MYH7) expression and its related mechanism.

    Amplification:

    Article Title: Development of a fluorescence isothermal recombinase polymerase amplification assay for rapid detection of feline calicivirus.
    Article Snippet: Feline calicivirus (FCV) is responsible for a highly contagious disease in domestic cats.. FCV may cause multiple symptoms and even death to the infected cats.. A simple and cost-effective real-time RPA assay was developed for rapid detection of FCV in clinical samples.



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